Efficient Metabolism of Benzo(a)pyrene at Nanomolar Concentrations by Intact Murine Hepatoma Cells1

We have studied the metabolism of benzo(a)pyrene (BP) by intact mouse hepatoma cells, at nM concentrations of the carcinogen, using an assay in which we directly measure the rate of BP fluorescence disappearance. The rate of BP metab olism is half-maximal, at limiting cell dilution, when the concen tration of BP is about 4 nM. This apparent Kmfor BP metabolism is much lower than those reported previously for several rea sons, (a) Partitioning of BP into cells markedly influences kinetic measurements, and we account for these effects, (b) Enzyme inducers can competitively inhibit BP metabolism and thus may introduce artifacts into kinetic measurements, (c) Under the conditions of this assay, phenolic BP metabolites are produced but do not accumulate, due to their further metabo lism; therefore, assays of BP metabolism which measure the production of phenols, such as the commonly used aryl hydro carbon hydroxylase assay, may markedly underestimate the rate of BP metabolism when intact cells and low substrate concentrations are used. Our results show that cells can effi ciently metabolize BP when exposed to BP concentrations similar to those present in the environment.